Extraction of polysaccharide from Spirulina platensis - advantage of freeze-thaw method

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C-PHYCOCYANIN EXTRACTION FROM Spirulina platensis WET BIOMASS

C-Phycocyanin is a natural blue dye used in food and pharmaceutical industry. In the present study, a simple and efficient method to extract C-phycocyanin from Spirulina platensis wet biomass is reported. The extractions were carried out using six different methods, including chemical (organic and inorganic acid treatment), physical (freezing and thawing, sonication, homogenization) and enzymat...

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Application of an Ultrafine Shearing Method for the Extraction of C-Phycocyanin from Spirulina platensis.

Cell disruption is an important step during the extraction of C-phycocyanin from Spirulina platensis. An ultrafine shearing method is introduced and combined with soaking and ultrasonication to disrupt the cell walls of S. platensis efficiently and economically. Five kinds of cell disruption method, including soaking, ultrasonication, freezing-thawing, soaking-ultrafine shearing and soaking-ult...

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Effects of structural modification of calcium spirulan, a sulfated polysaccharide from Spirulina platensis, on antiviral activity.

Calcium ion binding with the anionic part of a molecule was replaced with various metal cations and their inhibitory effects on the replication of herpes simplex virus type 1 were evaluated. Replacement of calcium ion with sodium and potassium ions maintained the antiviral activity while divalent and trivalent metal cations reduced the activity. Depolymerization of sodium spirulan with hydrogen...

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Inhibitory effects of polysaccharide extract from Spirulina platensis on corneal neovascularization

PURPOSE To assess the effects of polysaccharide extract from Spirulina platensis (PSP) on corneal neovascularization (CNV) in vivo and in vitro. METHODS PSP was extracted from dry powder of Spirulina platensis. Its anti-angiogenic activity was evaluated in the mouse corneal alkali burn model after topical administration of PSP four times daily for up to seven days. Corneal samples were proces...

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Stable Isolation of Phycocyanin from Spirulina platensis Associated with High-Pressure Extraction Process

A method for stably purifying a functional dye, phycocyanin from Spirulina platensis was developed by a hexane extraction process combined with high pressure. This was necessary because this dye is known to be very unstable during normal extraction processes. The purification yield of this method was estimated as 10.2%, whose value is 3%-5% higher than is the case from another conventional sep...

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ژورنال

عنوان ژورنال: Vietnam Journal of Biotechnology

سال: 2021

ISSN: 1811-4989,1811-4989

DOI: 10.15625/1811-4989/18/4/16087